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Oct 01, 2015· Cellular assays for evaluation of antioxidant activity Development of the in vitro chemical assays and use of biologically relevant macromolecule model systems have enabled evaluation of intrinsic antioxidant activity of a great number of synthetic bioactive compounds and natural extracts.
The measurement of antioxidant activity using biologically relevant assays is important to screen fruits, vegetables, natural products, and dietary supplements for potential health benefits. The cellular antioxidant activity (CAA) assay quantifies antioxidant activity using a cell culture model and was developed to meet the need for a more biologically representative method than the popular ...
Comparative study on the phytochemical profiles and cellular antioxidant activity of phenolics extracted from barley malts processed under different roasting temperatures Y. Chen, J. Huang, J. Hu, R. Yan and X. Ma, Food Funct., 2019, 10, 2176 DOI ...
The objective of this study was to evaluate effects of hazel leaves, rich in tannins, on blood antioxidant activity, cellular immune response, and heart beat parameters in sheep. Four experimental pellets were produced by mixing alfalfa and hazel leaves in different proportions, including alfalfa alone as a control, 30% and 60% of hazel leaves ...
Sep 29, 2007· A cellular antioxidant activity (CAA) assay for quantifying the antioxidant activity of phytochemicals, food extracts, and dietary supplements has been developed. Dichlorofluorescin is a probe that is trapped within cells and is easily oxidized to fluorescent dichlorofluorescein (DCF). The method measures the ability of compounds to prevent the formation of DCF by 2,2′-azobis(2 ...
Measurement of antioxidant activity using biologically relevant assays is important in the screening of fruits for potential health benefits. The cellular antioxidant activity (CAA) assay quantifies antioxidant activity in cell culture and was developed to meet the need for a more biologically representative method than the popular chemistry antioxidant capacity measures.
Excess ROS must be promptly eliminated from the cell by a variety of antioxidant defense mechanisms. Cellular antioxidant enzymes and other redox molecules serve to counterbalance ROS generated in the cell. We offer assays to measure the activity of specific antioxidants: catalase, superoxide dismutase and glutathione. Additionally, you may measure the antioxidant capacity of certain ...
Mar 16, 2020· Antioxidants had a growing interest owing to their protective roles in food and pharmaceutical products against oxidative deterioration and in the body and against oxidative stress-mediated pathological processes. Screening of antioxidant properties of plants and plant-derived compounds requires appropriate methods, which address the mechanism of antioxidant activity and …
May 29, 2018· Antioxidants can protect against the cell damage that free radicals cause, known as oxidative stress. Activities and processes that can lead to oxidative stress include:. mitochondrial activity
Aug 30, 2008· Measurement of antioxidant activity using biologically relevant assays is important in the screening of fruits for potential health benefits. The cellular antioxidant activity (CAA) assay quantifies antioxidant activity in cell culture and was developed to meet the need for a more biologically representative method than the popular chemistry antioxidant capacity measures.
Abstract Rosemary ethanol extract (REE) from Rosmarinus officinalis was identified by LC‐ESI‐MS/MS and 12 compounds were found. Among them, rosmarinic acid (389.78 μg/mg in REE), luteolin‐3ʹ‐O‐gluc...
Cellular Antioxidant Assay Kit (ab242300) is a cell-based assay for measuring antioxidant activity within adherent cells. Cells are first cultured in a 96-well black fluorescence cell …
Phenolic profiles and cellular antioxidant activity of longan pulp of 24 representative Chinese cultivars Ruifen Zhang a†, Sher Ali Khan †, Yaosheng Lina, Dongliang Guob, Xuewen Panb, Lei Liu, Zhencheng Wei a, Yan Zhang, Yuanyuan Denga, and Mingwei Zhang aKey Laboratory of Functional Foods, Ministry of Agriculture, Guangdong Key Laboratory of Agricultural Products
Dec 09, 2013· The human breast adenocarcinoma cell line, MCF-7, and the human mammary epithelial cell line, 184B5, were used in the anti-proliferation study. Assays of antioxidant enzymes and caspase activities, cell cycle analysis, and inhibition of cell migration were performed using MCF-7 cells. Mouse fibroblast cells, 3 T3-L1, were used in the comet assay.
To determine whether luteolin-induced HT-29 cell apoptosis was associated with the antioxidant properties of luteolin, we examined the antioxidant activity of luteolin. Decreased activity and expression of mitochondrial MnSOD was reported in certain colorectal carcinomas, probably accounting for increased superoxide radical production ( 42 ).
The highest antioxidant activity was found in the whole shiitake powder and the same decreasing trend (whole > cap > stem) was exhibited as in the ABTS determination with the stem having a value of 4.30 ± 0.29 μM TE/g (Fig. 2G). The whole shiitake powder had an antioxidant activity which was 19-fold higher than the wheat flour.
Mar 12, 2018· The antioxidant content of artichokes can vary, depending on how they are prepared. Boiling artichokes may raise their antioxidant content by eight times, and steaming them may raise it …
Production of protein hydrolysate as nutraceuticals is typically based on the activity of the hydrolysate, which might not yield the optimal activity under physiological condition due to structural modification of peptides upon gastrointestinal (GI) digestion. This study systematically compared the chemical and cellular antioxidant activities of the in vitro digesta of tilapia protein and its ...
Cellular Antioxidant Activity (CAA) Assay for Assessing Antioxidants, Foods, and Dietary Supplements
Cellular antioxidant activity (CAA) In the CAA assay, it was also found that the conversion treatment could significantly improve the antioxidant activity of chokeberry pomace (Fig. 5C). After conversion treatment, the cell antioxidant activity of the chokeberry pomace increased from 288.8 to 1378.0 (μmol QE/100g FW), which was increased 4.77 ...
The OxiSelect™ Cellular Antioxidant Assay Kit is a cell-based assay for measuring the activity of an exogenous antioxidant compound within adherent cells. Cells are first cultured in a 96-well black fluorescence cell culture plate until confluent. Then the cells are pre-incubated with a cell-permeable DCFH-DA fluorescence probe dye and the bioflavonoid Quercetin, or the antioxidant sample ...
The cell viability in sample groups was shown as the percentage compared with that of NEG. 2.2.11.1. Cellular antioxidant activity (CAA) assay. CAA was evaluated using the commercial assay kit based on the intracellular oxidation of dichlorodihydrofluorescein diacetate (DCFH-DA) (Beyotime Institute of Biotechnology, Beijing, China).
Sep 01, 2008· The cellular antioxidant activity (CAA) assay quantifies antioxidant activity in cell culture and was developed to meet the need for a more biologically representative method than …
Abstract The aim of our study was to determine the antioxidant activities, cytotoxicity and proliferative properties in Stevia rebaudiana leaves and stems. Leaves extracts exhibited a higher antioxidant activity than stems extract, through oxygen radical absorbance capacity (ORAC) and cellular antioxidant activity (CAA) assays. Stevioside and rebaudioside A, the main sweetening metabolites …
The Cellular Antioxidant Assay Kit (Green Fluorescence) is a cell-based assay for measuring antioxidant activity within a cell in a standard cell culture environment. This environment accounts for the temperature, pH, uptake, metabolism and efficacy of antioxidants in a whole cell. The assay employs the cell-permeable fluorogenic probe dye 2 ...
The measurement of antioxidant activity using biologically relevant assays is important to screen fruits, vegetables, natural products, and dietary supplements for potential health benefits. The cellular antioxidant activity (CAA) assay quantifies antioxidant activity using a cell culture model and was developed to meet the need for a more biologically representative method than the popular...
Mar 19, 2014· The antiproliferative activities against Caco-2 cell line didn't necessarily be associated with a high antioxidant capacity and the total phenolic concentration, possibly, qualitative and ...
Jul 29, 2019· This fat-soluble antioxidant plays a critical role in protecting cell membranes against oxidative damage. Flavonoids. This group of plant antioxidants has many beneficial health effects ( 18 ).
The cellular antioxidant activities of bract and leaf extracts (half maximal effective concentration (EC₅₀) = 26.6–124.1 mg L⁻¹) are better than or similar to that of a commercial leaf extract, and are related to the dicaffeoylquinic acid levels, particularly to 1,5-dicaffeoylquinic acid.